Illumina NovaSeq 6000
Sequencing Platforms
The NovaSeq 6000 System is the latest high throughput sequencing instrument released by Illumina. With up to 6Tb of data and 20 Billion paired-end reads made possible by the Illumina NovaSeq…the possibilities are endless. Not only does the NovaSeq increase our data output capabilities, but it is also incredibly cost effective. What was once unthinkable for many research groups can now become commonplace. From whole genome sequencing to high-coverage exome sequencing and even cancer-related variant detection, the Illumina NovaSeq has the power and affordability to make all of this possible.
While previous sequencing instruments such as the HiSeq X or HiSeq 4000 may have been limited to large studies or whole genome and exome sequencing applications, this is not the case with the NovaSeq 6000. The NovaSeq is one of the most flexible and scalable instruments currently available. By utilizing four different run types, we are able to provide our customers the necessary flexibility to sequence smaller bacterial genome projects just as easily as we can sequence whole human genome studies, and all for an affordable price.
| Sequencing Output Per Flow Cell | ||||
| NovaSeq 6000 System | ||||
| Flow Cell Type | SP | S1 | S2 | S4 |
| 2 × 50 bp | 65–80 Gb | 134–167 Gb | 333–417 Gb | N/A |
| 2 × 100 bp | N/A | 266–333 Gb | 667–833 Gb | 1600–2000 Gb |
| 2 × 150 bp | 200–250 Gb | 400–500 Gb | 1000–1250 Gb | 2400–3000 Gb |
| 2 x 250 bp | 325-400 Gb | N/A | N/A | N/A |
| Reads Passing Filter Per Flow Cell | ||||
| NovaSeq 6000 System | ||||
| Flow Cell Type | SP | S1 | S2 | S4 |
| Single-end Reads | 650–800 M | 1.3–1.6 B | 3.3 B–4.1 B | 8-10 B |
| Paired-end Reads | 1.3–1.6 B | 2.6–3.2 B | 6.6–8.2 B | 16–20 B |
| Estimated Sample Throughput for Key Applications | ||||
| NovaSeq 6000 System | ||||
| Flow Cell Type | SP | S1 | S2 | S4 |
| Human Genomes per Run | ~4 | ~8 | ~20 | ~48 |
| Exomes per Run | ~40 | ~80 | ~200 | ~500 |
| Transcriptomes per Run | ~32 | ~64 | ~164 | ~400 |